Picky parameter settings for this computation: maximum_oligo_size = 70 minimum_oligo_size = 50 maximum_match_len = 16 minimum_match_len = 10 maximum_gc_content = 70 minimum_gc_content = 30 candidates_per_gene = 5 probes_per_gene = 1 minimum_similarity = 75 minimum_temp_separation = 10 DNA_concentration = 1 Salt_concentration = 75 single_strand_only = 0 Job started on Fri 13 May 2005 09:34:37 AM CDT Step 1 of 6: Constructing the suffix array... 12238 target sequences. 12238 total sequences. 23015888 total DNA bases. 16380 is the longest sequence length. Step 2 of 6: Computing invert and lcp arrays... 5749 is the longest common overlap region length among sequences. 98.21 is the average common overlap region length among sequences. Step 3 of 6: Generating unique probe candidates... 2 CPU(s) used in this computation; step 3 is slower, be patient.... 6 sequences have no unique probe due to short overlaps with others. 3779 sequences have no unique probe due to long overlaps with others. 106 sequences have no good probe candidates due to temperature limit. 37467 initial unique probe candidates detected. Step 4 of 6: Identifying long common overlap regions... 22047 potentially sharable long common overlap regions identified. 5552 sequences hosting long common overlap regions identified. Step 5 of 6: Generating shared probe candidates... 2 CPU(s) used in this computation; step 5 is also slow, be patient... 35 host sequences were not useful due to short overlaps. 453 host sequences were taken by the other host sequences. 444 host sequences produce no probes due to temperature limit. 24882 additional sharable probe candidates detected. Step 6 of 6: Determining the optimal oligo set... 749 sequences/regions without probe at 63.24 C, 223 below, 526 above. 778 sequences/regions without probe at 64.05 C, 358 below, 420 above. 816 sequences/regions without probe at 64.55 C, 453 below, 363 above. 749 sequences/regions without probe at 10.00 separation temperature. 8213 unique oligo probes designed for 8213 sequences. 2479 shared oligo probes designed for 3896 additional sequences. 55 maximum number of sequences sharing the same oligo probe. 2.46 average number of sequences sharing the same oligo probe. 58.24 C is the maximum estimated probe-nontarget temperature. 68.28 C is the minimum estimated probe-target temperature. 63.24 C is the optimal estimated experiment temperature. 10692 total oligo probes designed for 12109 sequences. Job completed on Fri 13 May 2005 11:50:30 AM CDT Total time elapsed 02h:15m:53s.