Picky parameter settings for this computation: maximum_oligo_size = 70 minimum_oligo_size = 50 maximum_match_len = 15 minimum_match_len = 10 maximum_gc_content = 70 minimum_gc_content = 30 candidates_per_gene = 5 probes_per_gene = 1 minimum_similarity = 75 minimum_temp_separation = 20 DNA_concentration = 1 Salt_concentration = 75 single_strand_only = 0 Job started on Fri 20 May 2005 07:26:07 AM CDT Step 1 of 6: Constructing the suffix array... 9518 target sequences. 9518 total sequences. 10749024 total DNA bases. 16380 is the longest sequence length. Step 2 of 6: Computing invert and lcp arrays... 5295 is the longest common overlap region length among sequences. 35.36 is the average common overlap region length among sequences. Step 3 of 6: Generating unique probe candidates... 2 CPU(s) used in this computation; step 3 is slower, be patient.... 2972 sequences have no unique probe due to short overlaps with others. 2275 sequences have no unique probe due to long overlaps with others. 65 sequences have no good probe candidates due to temperature limit. 11018 initial unique probe candidates detected. Step 4 of 6: Identifying long common overlap regions... 3172 potentially sharable long common overlap regions identified. 1569 sequences hosting long common overlap regions identified. Step 5 of 6: Generating shared probe candidates... 2 CPU(s) used in this computation; step 5 is also slow, be patient... 476 host sequences were not useful due to short overlaps. 728 host sequences were taken by the other host sequences. 60 host sequences produce no probes due to temperature limit. 644 additional sharable probe candidates detected. Step 6 of 6: Determining the optimal oligo set... 281 sequences/regions without probe at 56.63 C, 100 below, 181 above. 277 sequences/regions without probe at 56.71 C, 104 below, 173 above. 434 sequences/regions without probe at 57.84 C, 316 below, 118 above. 290 sequences/regions without probe at 56.95 C, 133 below, 157 above. 277 sequences/regions without probe at 20.00 separation temperature. 3983 unique oligo probes designed for 3983 sequences. 216 shared oligo probes designed for 320 additional sequences. 7 maximum number of sequences sharing the same oligo probe. 2.19 average number of sequences sharing the same oligo probe. 46.71 C is the maximum estimated probe-nontarget temperature. 66.71 C is the minimum estimated probe-target temperature. 56.71 C is the optimal estimated experiment temperature. 4199 total oligo probes designed for 4303 sequences. Job completed on Fri 20 May 2005 07:30:46 AM CDT Total time elapsed 00h:04m:39s.