Picky parameter settings for this computation: maximum_oligo_size = 70 minimum_oligo_size = 50 maximum_match_len = 17 minimum_match_len = 10 maximum_gc_content = 70 minimum_gc_content = 30 candidates_per_gene = 5 probes_per_gene = 1 minimum_similarity = 75 minimum_temp_separation = 10 DNA_concentration = 1 Salt_concentration = 75 single_strand_only = 0 Job started on Wed 18 May 2005 04:54:50 PM CDT Step 1 of 6: Constructing the suffix array... 58579 target sequences. 58579 total sequences. 39022169 total DNA bases. 8251 is the longest sequence length. Step 2 of 6: Computing invert and lcp arrays... 3266 is the longest common overlap region length among sequences. 24.32 is the average common overlap region length among sequences. Step 3 of 6: Generating unique probe candidates... 2 CPU(s) used in this computation; step 3 is slower, be patient.... 734 sequences have no unique probe due to short overlaps with others. 5791 sequences have no unique probe due to long overlaps with others. 1244 sequences have no good probe candidates due to temperature limit. 189712 initial unique probe candidates detected. Step 4 of 6: Identifying long common overlap regions... 24846 potentially sharable long common overlap regions identified. 13071 sequences hosting long common overlap regions identified. Step 5 of 6: Generating shared probe candidates... 2 CPU(s) used in this computation; step 5 is also slow, be patient... 1037 host sequences were not useful due to short overlaps. 2572 host sequences were taken by the other host sequences. 5001 host sequences produce no probes due to temperature limit. 8156 additional sharable probe candidates detected. Step 6 of 6: Determining the optimal oligo set... 5880 sequences/regions without probe at 63.96 C, 720 below, 5160 above. 5780 sequences/regions without probe at 64.13 C, 784 below, 4996 above. 5751 sequences/regions without probe at 64.21 C, 817 below, 4934 above. 5579 sequences/regions without probe at 64.64 C, 1005 below, 4574 above. 5515 sequences/regions without probe at 64.82 C, 1096 below, 4419 above. 5461 sequences/regions without probe at 64.99 C, 1194 below, 4267 above. 5428 sequences/regions without probe at 65.07 C, 1231 below, 4197 above. 5413 sequences/regions without probe at 65.25 C, 1339 below, 4074 above. 5407 sequences/regions without probe at 65.29 C, 1358 below, 4049 above. 5412 sequences/regions without probe at 65.43 C, 1463 below, 3949 above. 5375 sequences/regions without probe at 65.56 C, 1541 below, 3834 above. 5356 sequences/regions without probe at 65.73 C, 1649 below, 3707 above. 5336 sequences/regions without probe at 65.91 C, 1750 below, 3586 above. 5322 sequences/regions without probe at 66.09 C, 1868 below, 3454 above. 5360 sequences/regions without probe at 66.35 C, 2072 below, 3288 above. 5358 sequences/regions without probe at 66.36 C, 2081 below, 3277 above. 5392 sequences/regions without probe at 66.56 C, 2250 below, 3142 above. 5427 sequences/regions without probe at 66.76 C, 2421 below, 3006 above. 5521 sequences/regions without probe at 67.01 C, 2682 below, 2839 above. 5606 sequences/regions without probe at 67.16 C, 2831 below, 2775 above. 5322 sequences/regions without probe at 10.00 separation temperature. 46973 unique oligo probes designed for 46973 sequences. 1647 shared oligo probes designed for 1886 additional sequences. 7 maximum number of sequences sharing the same oligo probe. 2.14 average number of sequences sharing the same oligo probe. 61.08 C is the maximum estimated probe-nontarget temperature. 71.09 C is the minimum estimated probe-target temperature. 66.09 C is the optimal estimated experiment temperature. 48620 total oligo probes designed for 48859 sequences. Job completed on Thu 19 May 2005 01:03:52 AM CDT Total time elapsed 08h:09m:02s.