Picky parameter settings for this computation: maximum_oligo_size = 70 minimum_oligo_size = 50 maximum_match_len = 16 minimum_match_len = 10 maximum_gc_content = 70 minimum_gc_content = 30 candidates_per_gene = 5 probes_per_gene = 1 minimum_similarity = 75 minimum_temp_separation = 10 DNA_concentration = 1 Salt_concentration = 75 single_strand_only = 0 Job started on Fri 20 May 2005 08:43:44 AM CDT Step 1 of 6: Constructing the suffix array... 58579 target sequences. 58579 total sequences. 39022169 total DNA bases. 8251 is the longest sequence length. Step 2 of 6: Computing invert and lcp arrays... 3266 is the longest common overlap region length among sequences. 24.32 is the average common overlap region length among sequences. Step 3 of 6: Generating unique probe candidates... 4 CPU(s) used in this computation; step 3 is slower, be patient.... 1631 sequences have no unique probe due to short overlaps with others. 7467 sequences have no unique probe due to long overlaps with others. 1099 sequences have no good probe candidates due to temperature limit. 170333 initial unique probe candidates detected. Step 4 of 6: Identifying long common overlap regions... 24624 potentially sharable long common overlap regions identified. 13012 sequences hosting long common overlap regions identified. Step 5 of 6: Generating shared probe candidates... 4 CPU(s) used in this computation; step 5 is also slow, be patient... 1718 host sequences were not useful due to short overlaps. 3688 host sequences were taken by the other host sequences. 2938 host sequences produce no probes due to temperature limit. 8630 additional sharable probe candidates detected. Step 6 of 6: Determining the optimal oligo set... 5651 sequences/regions without probe at 63.82 C, 713 below, 4938 above. 5607 sequences/regions without probe at 63.94 C, 760 below, 4847 above. 5544 sequences/regions without probe at 64.08 C, 823 below, 4721 above. 5405 sequences/regions without probe at 64.39 C, 953 below, 4452 above. 5251 sequences/regions without probe at 64.86 C, 1187 below, 4064 above. 5221 sequences/regions without probe at 65.02 C, 1272 below, 3949 above. 5204 sequences/regions without probe at 65.07 C, 1297 below, 3907 above. 5199 sequences/regions without probe at 65.25 C, 1437 below, 3762 above. 5201 sequences/regions without probe at 65.39 C, 1546 below, 3655 above. 5160 sequences/regions without probe at 65.57 C, 1665 below, 3495 above. 5152 sequences/regions without probe at 65.82 C, 1832 below, 3320 above. 5184 sequences/regions without probe at 66.08 C, 2032 below, 3152 above. 5209 sequences/regions without probe at 66.25 C, 2171 below, 3038 above. 5224 sequences/regions without probe at 66.36 C, 2257 below, 2967 above. 5278 sequences/regions without probe at 66.56 C, 2457 below, 2821 above. 5344 sequences/regions without probe at 66.76 C, 2638 below, 2706 above. 5480 sequences/regions without probe at 67.00 C, 2895 below, 2585 above. 5383 sequences/regions without probe at 66.82 C, 2707 below, 2676 above. 5152 sequences/regions without probe at 10.00 separation temperature. 44444 unique oligo probes designed for 44444 sequences. 1984 shared oligo probes designed for 2317 additional sequences. 5 maximum number of sequences sharing the same oligo probe. 2.13 average number of sequences sharing the same oligo probe. 60.82 C is the maximum estimated probe-nontarget temperature. 70.83 C is the minimum estimated probe-target temperature. 65.82 C is the optimal estimated experiment temperature. 46428 total oligo probes designed for 46761 sequences. Job completed on Fri 20 May 2005 11:14:12 AM CDT Total time elapsed 02h:30m:28s.