Picky parameter settings for this computation: maximum_oligo_size = 70 minimum_oligo_size = 50 maximum_match_len = 15 minimum_match_len = 10 maximum_gc_content = 70 minimum_gc_content = 30 candidates_per_gene = 5 probes_per_gene = 1 minimum_similarity = 75 minimum_temp_separation = 20 DNA_concentration = 1 Salt_concentration = 75 single_strand_only = 0 Job started on Wed 18 May 2005 02:27:14 PM CDT Step 1 of 6: Constructing the suffix array... 58579 target sequences. 58579 total sequences. 39022169 total DNA bases. 8251 is the longest sequence length. Step 2 of 6: Computing invert and lcp arrays... 3266 is the longest common overlap region length among sequences. 24.32 is the average common overlap region length among sequences. Step 3 of 6: Generating unique probe candidates... 2 CPU(s) used in this computation; step 3 is slower, be patient.... 7422 sequences have no unique probe due to short overlaps with others. 13166 sequences have no unique probe due to long overlaps with others. 4210 sequences have no good probe candidates due to temperature limit. 78354 initial unique probe candidates detected. Step 4 of 6: Identifying long common overlap regions... 24250 potentially sharable long common overlap regions identified. 12914 sequences hosting long common overlap regions identified. Step 5 of 6: Generating shared probe candidates... 2 CPU(s) used in this computation; step 5 is also slow, be patient... 3013 host sequences were not useful due to short overlaps. 6352 host sequences were taken by the other host sequences. 1300 host sequences produce no probes due to temperature limit. 3081 additional sharable probe candidates detected. Step 6 of 6: Determining the optimal oligo set... 14082 sequences/regions without probe at 63.18 C, 5975 below, 8107 above. 14149 sequences/regions without probe at 63.36 C, 6269 below, 7880 above. 14203 sequences/regions without probe at 63.42 C, 6416 below, 7787 above. 14215 sequences/regions without probe at 63.46 C, 6456 below, 7759 above. 14240 sequences/regions without probe at 63.48 C, 6512 below, 7728 above. 14241 sequences/regions without probe at 63.50 C, 6537 below, 7704 above. 14285 sequences/regions without probe at 63.58 C, 6696 below, 7589 above. 14303 sequences/regions without probe at 63.61 C, 6753 below, 7550 above. 14313 sequences/regions without probe at 63.67 C, 6855 below, 7458 above. 14301 sequences/regions without probe at 63.68 C, 6867 below, 7434 above. 14332 sequences/regions without probe at 63.74 C, 6952 below, 7380 above. 14419 sequences/regions without probe at 63.93 C, 7292 below, 7127 above. 14376 sequences/regions without probe at 63.80 C, 7080 below, 7296 above. 14082 sequences/regions without probe at 20.00 separation temperature. 20978 unique oligo probes designed for 20978 sequences. 1015 shared oligo probes designed for 1515 additional sequences. 5 maximum number of sequences sharing the same oligo probe. 2.09 average number of sequences sharing the same oligo probe. 53.18 C is the maximum estimated probe-nontarget temperature. 73.19 C is the minimum estimated probe-target temperature. 63.18 C is the optimal estimated experiment temperature. 21993 total oligo probes designed for 22493 sequences. Job completed on Wed 18 May 2005 03:15:42 PM CDT Total time elapsed 00h:48m:28s.