Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 16
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Tue 10 May 2005 11:49:55 AM CDT
Step 1 of 6: Constructing the suffix array...
28205 target sequences.
28205 total sequences.
72748721 total DNA bases.
16380 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
16179 is the longest common overlap region length among sequences.
292.92 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
2 CPU(s) used in this computation; step 3 is slower, be patient....
86 sequences have no unique probe due to short overlaps with others.
6416 sequences have no unique probe due to long overlaps with others.
826 sequences have no good probe candidates due to temperature limit.
90417 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
22409 potentially sharable long common overlap regions identified.
8339 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
2 CPU(s) used in this computation; step 5 is also slow, be patient...
253 host sequences were not useful due to short overlaps.
2438 host sequences were taken by the other host sequences.
731 host sequences produce no probes due to temperature limit.
22572 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
7111 sequences/regions without probe at 63.98 C, 2276 below, 4835 above.
7071 sequences/regions without probe at 64.13 C, 2369 below, 4702 above.
7043 sequences/regions without probe at 64.32 C, 2510 below, 4533 above.
7037 sequences/regions without probe at 64.38 C, 2556 below, 4481 above.
7012 sequences/regions without probe at 64.47 C, 2605 below, 4407 above.
7031 sequences/regions without probe at 64.64 C, 2760 below, 4271 above.
7036 sequences/regions without probe at 64.79 C, 2920 below, 4116 above.
7049 sequences/regions without probe at 64.83 C, 2955 below, 4094 above.
7048 sequences/regions without probe at 64.90 C, 3026 below, 4022 above.
7132 sequences/regions without probe at 65.18 C, 3310 below, 3822 above.
7148 sequences/regions without probe at 65.23 C, 3365 below, 3783 above.
7226 sequences/regions without probe at 65.41 C, 3576 below, 3650 above.
7266 sequences/regions without probe at 65.57 C, 3742 below, 3524 above.
7012 sequences/regions without probe at 20.00 separation temperature.
16886 unique oligo probes designed for 16886 sequences.
2888 shared oligo probes designed for 5714 additional sequences.
24 maximum number of sequences sharing the same oligo probe.
2.67 average number of sequences sharing the same oligo probe.
54.47 C is the maximum estimated probe-nontarget temperature.
74.47 C is the minimum estimated probe-target temperature.
64.47 C is the optimal estimated experiment temperature.
19774 total oligo probes designed for 22600 sequences.
Job completed on Tue 10 May 2005 08:25:22 PM CDT
Total time elapsed 08h:35m:27s.