Picky parameter settings for this computation: maximum_oligo_size = 70 minimum_oligo_size = 50 maximum_match_len = 15 minimum_match_len = 10 maximum_gc_content = 70 minimum_gc_content = 30 candidates_per_gene = 5 probes_per_gene = 1 minimum_similarity = 75 minimum_temp_separation = 20 DNA_concentration = 1 Salt_concentration = 75 single_strand_only = 0 Job started on Tue 10 May 2005 10:11:58 AM CDT Step 1 of 6: Constructing the suffix array... 28205 target sequences. 28205 total sequences. 72748721 total DNA bases. 16380 is the longest sequence length. Step 2 of 6: Computing invert and lcp arrays... 16179 is the longest common overlap region length among sequences. 292.92 is the average common overlap region length among sequences. Step 3 of 6: Generating unique probe candidates... 4 CPU(s) used in this computation; step 3 is slower, be patient.... 2813 sequences have no unique probe due to short overlaps with others. 9979 sequences have no unique probe due to long overlaps with others. 1158 sequences have no good probe candidates due to temperature limit. 34648 initial unique probe candidates detected. Step 4 of 6: Identifying long common overlap regions... 21834 potentially sharable long common overlap regions identified. 8271 sequences hosting long common overlap regions identified. Step 5 of 6: Generating shared probe candidates... 4 CPU(s) used in this computation; step 5 is also slow, be patient... 651 host sequences were not useful due to short overlaps. 4543 host sequences were taken by the other host sequences. 359 host sequences produce no probes due to temperature limit. 5925 additional sharable probe candidates detected. Step 6 of 6: Determining the optimal oligo set... 8124 sequences/regions without probe at 63.71 C, 3355 below, 4769 above. 8147 sequences/regions without probe at 63.79 C, 3440 below, 4707 above. 8201 sequences/regions without probe at 63.92 C, 3568 below, 4633 above. 8194 sequences/regions without probe at 63.93 C, 3572 below, 4622 above. 8211 sequences/regions without probe at 63.99 C, 3640 below, 4571 above. 8217 sequences/regions without probe at 64.11 C, 3737 below, 4480 above. 8243 sequences/regions without probe at 64.23 C, 3838 below, 4405 above. 8252 sequences/regions without probe at 64.32 C, 3900 below, 4352 above. 8282 sequences/regions without probe at 64.46 C, 4048 below, 4234 above. 8337 sequences/regions without probe at 64.59 C, 4198 below, 4139 above. 8301 sequences/regions without probe at 64.49 C, 4084 below, 4217 above. 8124 sequences/regions without probe at 20.00 separation temperature. 7809 unique oligo probes designed for 7809 sequences. 1325 shared oligo probes designed for 3140 additional sequences. 24 maximum number of sequences sharing the same oligo probe. 2.64 average number of sequences sharing the same oligo probe. 53.71 C is the maximum estimated probe-nontarget temperature. 73.71 C is the minimum estimated probe-target temperature. 63.71 C is the optimal estimated experiment temperature. 9134 total oligo probes designed for 10949 sequences. Job completed on Tue 10 May 2005 10:31:55 AM CDT Total time elapsed 00h:19m:57s.