Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 15
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Wed 11 May 2005 01:59:34 PM CDT
Step 1 of 6: Constructing the suffix array...
26236 target sequences.
26236 total sequences.
32759147 total DNA bases.
15717 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
9948 is the longest common overlap region length among sequences.
92.75 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
4 CPU(s) used in this computation; step 3 is slower, be patient....
304 sequences have no unique probe due to short overlaps with others.
10027 sequences have no unique probe due to long overlaps with others.
316 sequences have no good probe candidates due to temperature limit.
53264 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
29239 potentially sharable long common overlap regions identified.
11246 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
4 CPU(s) used in this computation; step 5 is also slow, be patient...
296 host sequences were not useful due to short overlaps.
3636 host sequences were taken by the other host sequences.
670 host sequences produce no probes due to temperature limit.
16402 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
7527 sequences/regions without probe at 62.78 C, 2942 below, 4585 above.
7546 sequences/regions without probe at 62.96 C, 3123 below, 4423 above.
7584 sequences/regions without probe at 63.12 C, 3291 below, 4293 above.
7614 sequences/regions without probe at 63.20 C, 3371 below, 4243 above.
7605 sequences/regions without probe at 63.23 C, 3396 below, 4209 above.
7609 sequences/regions without probe at 63.29 C, 3450 below, 4159 above.
7686 sequences/regions without probe at 63.46 C, 3634 below, 4052 above.
7766 sequences/regions without probe at 63.63 C, 3852 below, 3914 above.
7776 sequences/regions without probe at 63.66 C, 3883 below, 3893 above.
7818 sequences/regions without probe at 63.76 C, 4002 below, 3816 above.
7527 sequences/regions without probe at 20.00 separation temperature.
12158 unique oligo probes designed for 12158 sequences.
3773 shared oligo probes designed for 6543 additional sequences.
11 maximum number of sequences sharing the same oligo probe.
2.44 average number of sequences sharing the same oligo probe.
52.78 C is the maximum estimated probe-nontarget temperature.
72.79 C is the minimum estimated probe-target temperature.
62.78 C is the optimal estimated experiment temperature.
15931 total oligo probes designed for 18701 sequences.
Job completed on Wed 11 May 2005 02:23:11 PM CDT
Total time elapsed 00h:23m:37s.