Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 15
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Mon 16 May 2005 11:32:41 AM CDT
Step 1 of 6: Constructing the suffix array...
28952 target sequences.
28952 total sequences.
36327482 total DNA bases.
16011 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
10281 is the longest common overlap region length among sequences.
79.57 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
2 CPU(s) used in this computation; step 3 is slower, be patient....
1856 sequences have no unique probe due to short overlaps with others.
5873 sequences have no unique probe due to long overlaps with others.
1366 sequences have no good probe candidates due to temperature limit.
62088 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
8946 potentially sharable long common overlap regions identified.
4950 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
2 CPU(s) used in this computation; step 5 is also slow, be patient...
713 host sequences were not useful due to short overlaps.
1502 host sequences were taken by the other host sequences.
342 host sequences produce no probes due to temperature limit.
7089 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
3959 sequences/regions without probe at 63.01 C, 1870 below, 2089 above.
3952 sequences/regions without probe at 63.13 C, 1958 below, 1994 above.
3954 sequences/regions without probe at 63.22 C, 2029 below, 1925 above.
3952 sequences/regions without probe at 20.00 separation temperature.
16407 unique oligo probes designed for 16407 sequences.
1890 shared oligo probes designed for 3744 additional sequences.
15 maximum number of sequences sharing the same oligo probe.
2.20 average number of sequences sharing the same oligo probe.
53.13 C is the maximum estimated probe-nontarget temperature.
73.13 C is the minimum estimated probe-target temperature.
63.13 C is the optimal estimated experiment temperature.
18297 total oligo probes designed for 20151 sequences.
Job completed on Mon 16 May 2005 12:36:08 PM CDT
Total time elapsed 01h:03m:27s.