Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 16
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Sat 14 May 2005 05:10:35 PM CDT
Step 1 of 6: Constructing the suffix array...
18962 target sequences.
18962 total sequences.
32217720 total DNA bases.
16380 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
16380 is the longest common overlap region length among sequences.
485.55 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
2 CPU(s) used in this computation; step 3 is slower, be patient....
18 sequences have no unique probe due to short overlaps with others.
6600 sequences have no unique probe due to long overlaps with others.
12 sequences have no good probe candidates due to temperature limit.
54641 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
6502 potentially sharable long common overlap regions identified.
4190 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
2 CPU(s) used in this computation; step 5 is also slow, be patient...
23 host sequences were not useful due to short overlaps.
271 host sequences were taken by the other host sequences.
119 host sequences produce no probes due to temperature limit.
19479 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
344 sequences/regions without probe at 64.97 C, 77 below, 267 above.
317 sequences/regions without probe at 66.71 C, 156 below, 161 above.
364 sequences/regions without probe at 68.22 C, 275 below, 89 above.
314 sequences/regions without probe at 66.95 C, 173 below, 141 above.
314 sequences/regions without probe at 10.00 separation temperature.
12143 unique oligo probes designed for 12143 sequences.
3411 shared oligo probes designed for 6670 additional sequences.
26 maximum number of sequences sharing the same oligo probe.
2.78 average number of sequences sharing the same oligo probe.
61.94 C is the maximum estimated probe-nontarget temperature.
71.97 C is the minimum estimated probe-target temperature.
66.95 C is the optimal estimated experiment temperature.
15554 total oligo probes designed for 18813 sequences.
Job completed on Sat 14 May 2005 08:39:33 PM CDT
Total time elapsed 03h:28m:58s.