Picky parameter settings for this computation: maximum_oligo_size = 70 minimum_oligo_size = 50 maximum_match_len = 15 minimum_match_len = 10 maximum_gc_content = 70 minimum_gc_content = 30 candidates_per_gene = 5 probes_per_gene = 1 minimum_similarity = 75 minimum_temp_separation = 20 DNA_concentration = 1 Salt_concentration = 75 single_strand_only = 0 Job started on Sun 15 May 2005 08:22:16 PM CDT Step 1 of 6: Constructing the suffix array... 18962 target sequences. 18962 total sequences. 32217720 total DNA bases. 16380 is the longest sequence length. Step 2 of 6: Computing invert and lcp arrays... 16380 is the longest common overlap region length among sequences. 485.55 is the average common overlap region length among sequences. Step 3 of 6: Generating unique probe candidates... 2 CPU(s) used in this computation; step 3 is slower, be patient.... 337 sequences have no unique probe due to short overlaps with others. 7096 sequences have no unique probe due to long overlaps with others. 157 sequences have no good probe candidates due to temperature limit. 43676 initial unique probe candidates detected. Step 4 of 6: Identifying long common overlap regions... 6465 potentially sharable long common overlap regions identified. 4186 sequences hosting long common overlap regions identified. Step 5 of 6: Generating shared probe candidates... 2 CPU(s) used in this computation; step 5 is also slow, be patient... 105 host sequences were not useful due to short overlaps. 491 host sequences were taken by the other host sequences. 114 host sequences produce no probes due to temperature limit. 15174 additional sharable probe candidates detected. Step 6 of 6: Determining the optimal oligo set... 2715 sequences/regions without probe at 64.43 C, 699 below, 2016 above. 2641 sequences/regions without probe at 64.67 C, 749 below, 1892 above. 2622 sequences/regions without probe at 64.72 C, 768 below, 1854 above. 2568 sequences/regions without probe at 64.90 C, 817 below, 1751 above. 2529 sequences/regions without probe at 65.03 C, 837 below, 1692 above. 2504 sequences/regions without probe at 65.21 C, 890 below, 1614 above. 2492 sequences/regions without probe at 65.39 C, 928 below, 1564 above. 2475 sequences/regions without probe at 65.68 C, 1032 below, 1443 above. 2441 sequences/regions without probe at 65.91 C, 1103 below, 1338 above. 2456 sequences/regions without probe at 66.22 C, 1234 below, 1222 above. 2441 sequences/regions without probe at 20.00 separation temperature. 9691 unique oligo probes designed for 9691 sequences. 2920 shared oligo probes designed for 6602 additional sequences. 26 maximum number of sequences sharing the same oligo probe. 2.80 average number of sequences sharing the same oligo probe. 55.91 C is the maximum estimated probe-nontarget temperature. 75.92 C is the minimum estimated probe-target temperature. 65.91 C is the optimal estimated experiment temperature. 12611 total oligo probes designed for 16293 sequences. Job completed on Sun 15 May 2005 09:24:55 PM CDT Total time elapsed 01h:02m:39s.