Picky parameter settings for this computation: maximum_oligo_size = 70 minimum_oligo_size = 50 maximum_match_len = 15 minimum_match_len = 10 maximum_gc_content = 70 minimum_gc_content = 30 candidates_per_gene = 5 probes_per_gene = 1 minimum_similarity = 75 minimum_temp_separation = 10 DNA_concentration = 1 Salt_concentration = 75 single_strand_only = 0 Job started on Fri 27 May 2005 11:40:41 AM CDT Step 1 of 6: Constructing the suffix array... 30935 target sequences. 30935 total sequences. 34783951 total DNA bases. 16380 is the longest sequence length. Step 2 of 6: Computing invert and lcp arrays... 16380 is the longest common overlap region length among sequences. 115.38 is the average common overlap region length among sequences. Step 3 of 6: Generating unique probe candidates... 2 CPU(s) used in this computation; step 3 is slower, be patient.... 2224 sequences have no unique probe due to short overlaps with others. 9639 sequences have no unique probe due to long overlaps with others. 149 sequences have no good probe candidates due to temperature limit. 59996 initial unique probe candidates detected. Step 4 of 6: Identifying long common overlap regions... 14531 potentially sharable long common overlap regions identified. 7546 sequences hosting long common overlap regions identified. Step 5 of 6: Generating shared probe candidates... 2 CPU(s) used in this computation; step 5 is also slow, be patient... 714 host sequences were not useful due to short overlaps. 3035 host sequences were taken by the other host sequences. 268 host sequences produce no probes due to temperature limit. 8710 additional sharable probe candidates detected. Step 6 of 6: Determining the optimal oligo set... 865 sequences/regions without probe at 62.92 C, 180 below, 685 above. 847 sequences/regions without probe at 63.55 C, 282 below, 565 above. 906 sequences/regions without probe at 64.37 C, 452 below, 454 above. 1007 sequences/regions without probe at 64.87 C, 611 below, 396 above. 928 sequences/regions without probe at 64.50 C, 486 below, 442 above. 847 sequences/regions without probe at 10.00 separation temperature. 18301 unique oligo probes designed for 18301 sequences. 2584 shared oligo probes designed for 3529 additional sequences. 16 maximum number of sequences sharing the same oligo probe. 2.27 average number of sequences sharing the same oligo probe. 58.55 C is the maximum estimated probe-nontarget temperature. 68.56 C is the minimum estimated probe-target temperature. 63.55 C is the optimal estimated experiment temperature. 20885 total oligo probes designed for 21830 sequences. Job completed on Fri 27 May 2005 12:22:26 PM CDT Total time elapsed 00h:41m:45s.