maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 15
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0
6343 target sequences.
6343 total sequences.
8959501 total DNA bases.
14733 is the longest sequence length.
36 sequences were dead due to short overlaps with others.
478 sequences were dead due to long overlaps with others.
66 sequences were dead with no good probe candidates.
26505 initial best probe candidates detected.
103 sequences without probe due to temperature conflicts.
5660 total oligo probes designed for 5660 sequences.
59.85 C is the optimal estimated experiment temperature.
total time elapsed 2925 seconds