maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 15
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0
25392 target sequences.
25392 total sequences.
52701574 total DNA bases.
16380 is the longest sequence length.
2594 sequences were dead due to short overlaps with others.
5184 sequences were dead due to long overlaps with others.
112 sequences were dead with no good probe candidates.
54745 initial best probe candidates detected.
1015 sequences without probe due to temperature conflicts.
16487 total oligo probes designed for 16487 sequences.
65.00 C is the optimal estimated experiment temperature.
total time elapsed 4276 seconds