maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 16
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0
26088 target sequences.
26088 total sequences.
64875065 total DNA bases.
16380 is the longest sequence length.
127 sequences were dead due to short overlaps with others.
5121 sequences were dead due to long overlaps with others.
603 sequences were dead with no good probe candidates.
88812 initial best probe candidates detected.
3673 sequences without probe due to temperature conflicts.
16564 total oligo probes designed for 16564 sequences.
64.48 C is the optimal estimated experiment temperature.
total time elapsed 57032 seconds