maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 16
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0
26088 target sequences.
26088 total sequences.
64875065 total DNA bases.
16380 is the longest sequence length.
127 sequences were dead due to short overlaps with others.
5121 sequences were dead due to long overlaps with others.
181 sequences were dead with no good probe candidates.
92026 initial best probe candidates detected.
606 sequences without probe due to temperature conflicts.
20053 total oligo probes designed for 20053 sequences.
66.54 C is the optimal estimated experiment temperature.
total time elapsed 57601 seconds