maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 15
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0
26088 target sequences.
26088 total sequences.
64875065 total DNA bases.
16380 is the longest sequence length.
2966 sequences were dead due to short overlaps with others.
7532 sequences were dead due to long overlaps with others.
901 sequences were dead with no good probe candidates.
38840 initial best probe candidates detected.
6057 sequences without probe due to temperature conflicts.
8632 total oligo probes designed for 8632 sequences.
63.55 C is the optimal estimated experiment temperature.
total time elapsed 3826 seconds