maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 15
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0
26088 target sequences.
26088 total sequences.
64875065 total DNA bases.
16380 is the longest sequence length.
2966 sequences were dead due to short overlaps with others.
7532 sequences were dead due to long overlaps with others.
69 sequences were dead with no good probe candidates.
44045 initial best probe candidates detected.
1320 sequences without probe due to temperature conflicts.
14201 total oligo probes designed for 14201 sequences.
65.97 C is the optimal estimated experiment temperature.
total time elapsed 3823 seconds