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maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 16
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0
9616 target sequences.
9616 total sequences.
10783114 total DNA bases.
16380 is the longest sequence length.
4 CPU used in this computation.
2601 sequences were dead due to short overlaps with others.
1500 sequences were dead due to long overlaps with others.
87 sequences were dead with no good probe candidates.
17555 initial best probe candidates detected.
223 sequences without probe at 56.41 C, 89 below, 134 above.
245 sequences without probe at 56.68 C, 127 below, 118 above.
223 sequences without probe due to temperature conflicts.
5205 total oligo probes designed for 5205 sequences.
46.38 C is the maximum estimated probe-nontarget temperature.
66.42 C is the minimum estimated probe-target temperature.
56.41 C is the optimal estimated experiment temperature.