maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 15
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0
9616 target sequences.
9616 total sequences.
10783114 total DNA bases.
16380 is the longest sequence length.
3556 sequences were dead due to short overlaps with others.
1732 sequences were dead due to long overlaps with others.
58 sequences were dead with no good probe candidates.
11191 initial best probe candidates detected.
219 sequences without probe due to temperature conflicts.
4051 total oligo probes designed for 4051 sequences.
56.71 C is the optimal estimated experiment temperature.
total time elapsed 570 seconds