maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 17
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 10
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0
9616 target sequences.
9616 total sequences.
10783114 total DNA bases.
16380 is the longest sequence length.
1052 sequences were dead due to short overlaps with others.
1209 sequences were dead due to long overlaps with others.
26 sequences were dead with no good probe candidates.
26769 initial best probe candidates detected.
140 sequences without probe due to temperature conflicts.
7189 total oligo probes designed for 7189 sequences.
55.40 C is the optimal estimated experiment temperature.
total time elapsed 14035 seconds