maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 16
minimum_match_len = 10
maximum_gc_content = 50
minimum_gc_content = 10
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0
9616 target sequences.
9616 total sequences.
10783114 total DNA bases.
16380 is the longest sequence length.
1978 sequences were dead due to short overlaps with others.
1425 sequences were dead due to long overlaps with others.
10 sequences were dead with no good probe candidates.
21244 initial best probe candidates detected.
86 sequences without probe due to temperature conflicts.
6117 total oligo probes designed for 6117 sequences.
55.73 C is the optimal estimated experiment temperature.
total time elapsed 4349 seconds