maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 16
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0
18513 target sequences.
18513 total sequences.
30348312 total DNA bases.
16380 is the longest sequence length.
29 sequences were dead due to short overlaps with others.
5880 sequences were dead due to long overlaps with others.
12 sequences were dead with no good probe candidates.
56134 initial best probe candidates detected.
175 sequences without probe due to temperature conflicts.
12417 total oligo probes designed for 12417 sequences.
64.99 C is the optimal estimated experiment temperature.
total time elapsed 7788 seconds