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maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 15
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0
18513 target sequences.
18513 total sequences.
30348312 total DNA bases.
16380 is the longest sequence length.
369 sequences were dead due to short overlaps with others.
6319 sequences were dead due to long overlaps with others.
151 sequences were dead with no good probe candidates.
45171 initial best probe candidates detected.
1651 sequences without probe due to temperature conflicts.
10023 total oligo probes designed for 10023 sequences.
65.91 C is the optimal estimated experiment temperature.
total time elapsed 2402 seconds