maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 16
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0
25447 target sequences.
25447 total sequences.
37829502 total DNA bases.
15628 is the longest sequence length.
79 sequences were dead due to short overlaps with others.
480 sequences were dead due to long overlaps with others.
283 sequences were dead with no good probe candidates.
112949 initial best probe candidates detected.
1089 sequences without probe due to temperature conflicts.
23516 total oligo probes designed for 23516 sequences.
61.69 C is the optimal estimated experiment temperature.
total time elapsed 15948 seconds