maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 15
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0
25447 target sequences.
25447 total sequences.
37829502 total DNA bases.
15628 is the longest sequence length.
4 CPU used in this computation.
2040 sequences were dead due to short overlaps with others.
1121 sequences were dead due to long overlaps with others.
906 sequences were dead with no good probe candidates.
67680 initial best probe candidates detected.
3686 sequences without probe at 62.89 C, 1897 below, 1789 above.
3672 sequences without probe at 62.78 C, 1846 below, 1826 above.
3715 sequences without probe at 62.49 C, 1688 below, 2027 above.
3697 sequences without probe at 62.62 C, 1753 below, 1944 above.
3672 sequences without probe due to temperature conflicts.
17708 total oligo probes designed for 17708 sequences.
52.78 C is the maximum estimated probe-nontarget temperature.
72.79 C is the minimum estimated probe-target temperature.
62.78 C is the optimal estimated experiment temperature.